Congcong Wang
Zhejiang University, ChinaTitle: Expression profiles and coexpression network analyses of long noncoding RNAs in brown adipose tissue of obesity-prone and obesityresistant mice fed a high-fat diet
Abstract
An obesity-prone
(OP) or an obesity-resistant (OR) phenotype exists under the same diet type,
including a high-fat diet (HFD). Brown adipose tissue (BAT) functions to
dissipate energy in response to cold exposure or overfeeding. Long noncoding
RNAs (LncRNAs) are an important class of pervasive genes involved in a variety
of biological functions. However, the potential biological functions of lncRNAs
related to OP or OR phenotype in BAT have not been fully understood. Here, we
constructed a high-fat diet-induced OP and OR mouse model. Transcriptome
analyses were performed to obtain the expression profiles of mRNAs and lncRNAs
in the BAT of the OP and OR mice. We detected 228 differentially expressed
lncRNAs and 1159 differentially expressed mRNAs between the OP and OR groups.
We further analysed differentially expressed Genes (DEGs) , using Gene set
enrichment, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes pathway
analysis. Co-expression analysis and target gene prediction were further
performed to determine the transcriptional regulatory relationship of the
differentially expressed lncRNAs and mRNAs between the OP and OR groups. The
expression levels of the candidate lncRNAs and mRNAs were validated in brown
adipocytes activated with norepinephrine bitartrate monohydrate (NE) and
Forskolin in vitro and in the BAT from cold-induced thermogenesis mice in vivo
by qPCR. Our data suggest that two candidate lncRNAs (A530050N04Rik and
4930528G23Rik) and four candidate mRNAs (Lep, Oxtr,Cars2, and Gmpr) were
involved in weight and metabolism regulation by cAMP and thermogenesis
pathways. Significant differences were detected between the transcriptomes of
the BAT from OP and OR mice. The differentially expressed lncRNAs and mRNAs
were enriched in two potential pathways involved in weight and metabolism
regulation. These results provide clues to the molecular mechanisms of
metabolic disorders as well as candidate biomarkers of risk for obesity
Biography
Congcong Wang is currently an M.S. at the Department of Nutrition and Food Hygiene, Zhejiang University School of Public Health. She receives research training under the supervision of Assoc.Prof. Fei Yang from Prof. Shankuan Zhu's team at Chronic Disease Research Institute and Zhejiang university-Stanford university Collaborative Laboratory for Health. Before joining Zhejiang University, she got her bachelor degree from the Department of Veterinary Medicine at Nanjing Agricultural University. Her research focuses on applying the multi-omics analysis to identify the molecular links underlying the obesity and related chronic diseases.
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