Riffat IqbalGovernment College University, Pakistan
Title: Correlation between Arsenic toxicity, AS3MT Gene polymorphism and onset of Type 2
Chronically, arsenic exposure in drinking water has become a serious public health issue. AS3MT gene is involved in the metabolism of arsenic, so a single nucleotide polymorphism in this gene may lead to the development of type 2 diabetes in arsenic-exposed areas. This study aimed to evaluate the association of the AS3MT gene with the development of type 2 diabetes in highly arsenic-exposed areas of Punjab, Pakistan. For this purpose, a total of 200 samples were collected 100 (50 diabetics and 50 non-diabetics) from Mustafa Abad and 100 (50 people with diabetes and 50 people with no diabetes) from Nishtar are two arsenic-exposed areas from Kasur and Lahore, respectively. rs11191439 was utilized as an influential variable to evaluate the association between arsenic metabolism and diabetes status to find a single nucleotide polymorphism in the AS3MT gene. We observed the arsenic level in drinking water of the arsenic-exposed selected areas 115.54 ± 1.23 and 96.88 ± 0.48, respectively. The As level in the urine of diabetics (98.54 ± 2.63 and 56.38 ± 12.66) were higher as compared to non-diabetics (77.58 ± 1.8 and 46.9 ± 8.95) of both affected areas, respectively. Correspondingly, the As level in the blood of diabetics (6.22 ± 0.12 and 5.26 ± 0.24) and non-diabetics (6.48 ± 0.08 and 5.49 ± 1.43) in the affected areas. Genotyping showed significant differences in the frequencies of alleles among cases and controls. Nevertheless, notable disparities in genotype distribution were observed in SNPs rs11191439 (C/T) (P=0.0013) and when comparing T2D patients and non-diabetic control subjects. The AS3MT gene shows a significant association with the affected people with diabetes living in arsenic-exposed areas.
To be updated