Congcong Wang
Hangzhou, Zhejiang University, ChinaTitle : Expression profiles and coexpression network analyses of long noncoding RNAs in brown adipose tissue of obesity-prone and obesity- resistant mice fed a high-fat diet
Abstract
An obesity-prone (OP) or an
obesity-resistant (OR) phenotype exists under the same diet type, including a
high-fat diet (HFD). Brown adipose tissue (BAT) functions to dissipate energy
in response to cold exposure or overfeeding. Long noncoding RNAs (LncRNAs) are
an important class of pervasive genes involved in a variety of biological
functions. However, the potential biological functions of lncRNAs related to OP
or OR phenotype in BAT have not been fully understood.
Here, we constructed a high-fat
diet-induced OP and OR mouse model. Transcriptome analyses were performed to
obtain the expression profiles of mRNAs and lncRNAs in the BAT of the OP and OR
mice. We detected 228 differentially expressed lncRNAs and 1159 differentially
expressed mRNAs between the OP and OR groups. We further analysed
differentially expressed Genes (DEGs) , using Gene set enrichment, Gene
Ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis.
Co-expression analysis and target gene prediction were further performed to
determine the transcriptional regulatory relationship of the differentially
expressed lncRNAs and mRNAs between the OP and OR groups. The expression levels
of the candidate lncRNAs and mRNAs were validated in brown adipocytes activated
with norepinephrine bitartrate monohydrate (NE) and Forskolin in vitro and in
the BAT from cold-induced thermogenesis mice in vivo by qPCR. Our data
suggest that two candidate lncRNAs (A530050N04Rik and 4930528G23Rik) and four
candidate mRNAs (Lep, Oxtr,Cars2, and Gmpr) were involved in weight and
metabolism regulation by cAMP and thermogenesis pathways.
Significant differences were
detected between the transcriptomes of the BAT from OP and OR mice. The
differentially expressed lncRNAs and mRNAs were enriched in two potential
pathways involved in weight and metabolism regulation. These results provide
clues to the molecular mechanisms of metabolic disorders as well as candidate
biomarkers of risk for obesity.
Biography
Congcong Wang is currently an
M.S. at the Department of Nutrition and Food Hygiene, Zhejiang University
School of Public Health. She receives research training under the supervision
of Assoc.Prof. Fei Yang from Prof. Shankuan Zhu's team at Chronic Disease
Research Institute and Zhejiang university-Stanford university Collaborative
Laboratory for Health. Before joining Zhejiang University, she got her bachelor
degree from the Department of Veterinary Medicine at Nanjing Agricultural
University. Her research focuses on applying the multi-omics analysis to
identify the molecular links underlying the obesity and related chronic
diseases.