Virtual Conference
Nutrition 2022

Pham kim Phuong

Saigon Technique University, VietNam

Title: Research method for determining Ethephon residues in fruit (Bananas ) on a high-pressure liquid chromatograph with MS/MS detector – LC/MS/MS when using Ethephon as a ripening agent.


Ethephon is not only has the effect in the stimulation of defoliation and but also makes fruits ripen quickly and evenly.  Ethephon pushes hard the process of fruit ripening from 1-2 days instead of 4-6 days as before. However, the residues of  Ethephon in the fruits will cause health problems such as damage to the liver, kidneys ....[1], [2]  The method used to determine Ethephon residues is high pressure liquid Chromatography Mass Spectrometry (LC-MS/MS) [3]. The limit of  detection (LOD) Etherphon is  0.92 mg /kg and  Limit of  quantitation is LOQ = 2,7mg/Kg . The linear range curve is from 0.96ppm to 96ppm with R2 = 0.999 repeatability.  The Study subjects are green bananas soaked in Ethephon solution with concentrations: 4.8%, 9.6% and 24%. Soaking time was from 1 day, 2 days 3, days and 4 days. Results obtained after 4 days of experiments showed that Ethephon content in banana increased proportionally with the concentration of ethephon in the solution. The highest increase was on day 1, measured at 90,5 ppm at soaked concentration 24% , and  42,2 ppm  at  9,6%  and finally  is 21,9ppm  at  soaked concentration of Ethephon 4,8%    . 
     Till the second, third, and fourth day, Ethephon  content in banana started to decrease.  Ethephon content in bananas  of  the fourth day  is  measured : 20ppm at soaked concentration  24%  , 9,6ppm at  soaked concentration  9,6%  and 3,3 ppm at soaked concentration   4,8%  of Ethephon  solution tank .  However, after 4 days, bananas soaked in Ethephon solution had blackened and become inedible in comparison with bananas soaked in the  Etherphon  solution only within 1 or 2 days, the banana still remained beautiful and edible.


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